Kevin Stadler, a two-time winner this season and son of Champions Tour player Craig Stadler, and Tyler Williamson share second place after opening rounds of 6-under 65 at Hillcrest Country Club.
Bolli had 230 yards at the par-5 16th and hit a 4-iron. His ball rolled in the cup for the first double-eagle in tournament history and the fifth of the season on the Nationwide Tour.
'That 4-iron was supposed to go to the middle of the green, but I pulled it a bit,' said Bolli, who won this year's Chattanooga Classic. 'The hole was way back left and no one would have aimed at that pin with a 4-iron.'
Bolli started well on Thursday with a tap-in birdie at the par-5 second hole. He added a 4-footer for birdie at the next, also a par-5, and made it three in a row with a 15-footer at the fourth.
He missed a 10-footer for par at the seventh to momentarily fall down the leaderboard, but a series of par saves around the turn kept him close to the top spot.
Bolli birdied the driveable, par-4 15th, but without a driver. He laid up short of the putting surface with a 6-iron, then hit a lob-wedge to 10 feet and drained the putt.
Bolli kept it going with birdies after the double-eagle. He knocked a pitching-wedge to 3 feet to set up birdie at the 17th, then ran home a 6-footer for birdie at the last for the first-round lead.
'I was trying not to think about what I was doing,' said Bolli, who is eighth on the tour's money list and is in good shape to earn his way on to the PGA Tour. 'I was just trying to hit good shots. Six under on the last four holes is silly. That was pretty ridiculous.'
Kyle Thompson and Scott Gump are tied for fourth place at 5-under-par 66. Jimmy Walker, also a two-time winner this year on tour, Steve Haskins, Robert Garrigus, Vance Veazey, Tom Scherrer, Andrew Morse, Andy Doeden, John Merrick, Rick Fehr and Carl Paulson are knotted in sixth place. They stand at 4-under-par 67.