Weekley's total of 128 tied the 36-hole record set by Joe Ogilvie in 2003 and matched last year by Troy Matteson.
Ken Duke, who won the Charity Pro-Am at The Cliffs earlier this year, also shot 63 to move into a share of second place at 15-under-par 129. He was joined there by 2002 U.S. Amateur winner Ricky Barnes (64).
The two first-round leaders are next on the leaderboard. Doug LaBelle shot 67 and is alone in fourth at minus-14, while 2000 U.S. Amateur champion Jeff Quinney posted a 68 and is alone in fifth at 13-under-par 131.
Weekley, whose best tour finish was a share of third earlier this year at the Chattanooga Classic, started on the 10th tee Friday and opened with back-to- back birdies to get to 9 under. After he parred the next four holes, Weekley birdied 16 and 18 to turn in minus-11.
Around the turn, Weekley birdied the first at Highland Springs Country Club. He tripped to his first bogey of the event at the second to slip back to 11 under.
Weekley, who turns 33-years-old on Sunday, caught fire down the stretch. He birdied the par-four fifth and came right back with a birdie on six. Weekley was not done.
He made it three in a row as he birdied the par-4 seventh. Weekley also birdied No. 8 and a birdie on the ninth made it five straight to close his round.
'I've just been focused on hitting the fairways,' Weekley stated. 'I'm hitting it solid, but there are a couple of shots I haven't mastered yet in my swing of thoughts. Basically out here it is going to be a putting contest. I hit a lot of good putts today.'
Duke also played the back nine first Friday. He poured in consecutive birdies from the 11th and again from the 15th to jump to 10 under. He picked up a birdie on 18 and came right back with a birdie on the first as well.
The 37-year-old Duke parred his next four holes. He ran off three birdies in a row from the sixth to move into a share of second place.
Barnes collected just one birdie over his first four holes, and that came on the par-5 11th. He birdied three straight from the 14th to get to 11 under.
After three pars in a row around the turn, Barnes carded four more birdies on the front nine to gain his share of second place.
'Boo and I had a lot of good looks today,' said Barnes, who was played with Weekley the first two rounds. 'We've fed off of each other the first two days. Our iron play has been pretty spot on.'
Nick Flanagan fired a 9-under 63 of his own. He stands at 12-under-par 132 and he shares sixth place with Josh Broadaway and Justin Bolli.
Athens Regional Foundation Classic winner Paul Gow shot 66 Friday. He leads a group of eight players at 11-under-par 133.
The cut line fell at 6-under-par 138 with 67 players moving on to the final two rounds. This is the sixth time in tour history a cut was minus-6. There were two events -- 1991 Dakota Dunes Open and 2004 Henrico County Open -- that had a cut at 7 under par for the lowest cut in tour history.