Returning to the course following a weather suspension Friday night -- and needing consecutive birdies to make the cut -- Woods ended his second round at the Funai Classic with bogey-par on his last two holes to miss the cut for the second time this year.
The world's No. 1 golfer ended with a 1-over 73 in the second round to finish at 3-under-par 141 and miss the cut by three strokes. He also missed the cut at the Byron Nelson Classic in May to snap a record-long streak of 142 consecutive cuts made.
Woods was all over the map during his second round, collecting six birdies but also posting a triple bogey, a double bogey and two more bogeys.
His final bogey came at the par-4 17th, where Woods' last tee shot before the weather suspension on Friday landed under a tree, leaving him with a difficult second shot from the rough.
'I had to play a great shot out of the trees and give myself a putt, which I didn't do,' said Woods. 'And I tried to hole a chip, and I didn't do that. I had to at least try to make a putt so I could eagle the last hole, and I didn't do that either.'
Woods' struggles came at the Walt Disney World Resort's Magnolia Course -- the event is using two courses for the first two rounds -- but another top player sputtered at the other host course.
World No. 2 Vijay Singh will have a rare weekend off after he managed just a 1-under 71 at the Palm Course to finish two rounds at 4-under-par 140 and miss the cut by two shots.
In his previous 11 starts since missing the cut at the Memorial during the first week of June, Singh had finished lower than 13th place just one time. Those 11 starts included eight top-10 placements and a victory at the Buick Open.
'Obviously [we] both didn't really play well,' Woods said, referring to himself and Singh. 'I can't speak for him, but I did not play well at all.'
The reality of Woods and Singh not making the cut stole some well-deserved attention from other players who had terrific rounds.
The leader heading into the third round will be Sweden's Carl Pettersson, who scorched the Palm Course for an 11-under 61 in his second round to get to 17- under-par 127.
That tied the 36-hole Disney record set by Chris DiMarco in 2002 and was good for a two-shot advantage over Tom Pernice Jr., who held the clubhouse lead overnight after a 10-under 62 in his second round on Friday.
Pettersson's bogey-free round included an amazing 11 birdies and featured a 10-hole stretch during which the Swede birdied eight holes. He finished his final three holes Saturday morning, going birdie-birdie at Nos. 7 and 8 before ending with a par at the ninth, his last hole.
'I was disappointed last night we couldn't finish,' said Pettersson, whose best-ever finish on the PGA Tour was a second place showing at the 2003 Buick Invitational. 'But I came out this morning and made two birdies. It was a nice feeling.'
With the iffy weather situation being what it is in Florida this weekend, Pettersson's score may be even a little better than it seems at the moment, if that's possible.
The PGA requires at least 54 holes for a tournament to be official, and thunderstorms are forecast for the remainder of the weekend.
'I think you go out there and do the best you can,' said Pettersson, responding to questions about approaching his next 18 holes like it could be the last round of the tournament.
'You can't predict what the weather is going to do. We might not have any rain for the next two days; we might have a ton.'
Kent Jones carded an 8-under 64 in his second round and is tied for third place with Geoff Ogilvy (66) at 14-under-par 130. Brandt Jobe shares fifth place with two other players at minus-13 after a second-round, 10-under 62. He is joined there by Steve Lowery (67) and Tim Clark (65).
Gallery darling Jason Gore is at minus-12 after a second-round 67. He shares eighth place with Stephen Leaney, who shot an 8-under 64.
The 6 under cut line matched a PGA record for lowest in relation to par.