Gustafson, who became the first woman to play on the Japan Golf Tour on Thursday, had two birdies, five bogeys and a double bogey on the Ibusuki Golf Club course Friday for a total of 7-over 151.
The Swede is a four-time winner on the LPGA Tour.
Japan's Katsumune Imai carded his second straight 65, including a hole in one on the par-3 fourth, to widen his lead to four strokes with a 36-hole total of 14-under 130. India's Jyoti Randhawa was in second place.
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