To be successful, 606 golfers will have to get round the Torrance course at St. Andrews Bay in Scotland on June 8.
Money raised from the 50 ($75)-a-head entry fee will go towards the 10 million ($15 million) cost of Scotland's second hospice for terminally ill children, to be built at Balloch on Loch Lomond.
The record for most rounds completed in a single day currently stands at 605 and was set at the Rhodes Ranch Golf Club in Las Vegas, Nev., on June 21, 1998.
The Scottish attempt will tee off at 3:30 a.m. The last four-ball is set to go at 7:30 p.m., with a quartet teeing off every six minutes between those times.
Although 'only' 606 participants are needed to break the record, organizers are aiming to get 650 around the course to make the feat more difficult to overtake