Matteson, who won the Virginia Beach Open two weeks ago, fired a 6-under 65 and is through 36 holes at nine-under-par 133. Robert Garrigus shot a 3-under 68 in Saturday's second round and is alone in third place at 7-under-par 135.
Since no golf was played on Friday due to inclement weather, the second round was contested on Saturday. The plan is for 36 holes on Sunday.
Bowden began his second round on the back nine at the TPC at Wakefield Plantation and broke into red figures with a birdie at the 10th. He added birdies at both par-5s on the back nine, 13 and 18.
Bowden dropped a shot to par at the second, but reclaimed the lost stroke with a birdie at the fourth. He bogeyed No. 8, but still had enough to take his first 36-hole lead on the Nationwide Tour.
'I just didn't make the putts today that I made on Thursday,' said the 36- year-old Bowden, who is a two-time winner on the Nationwide Tour. 'I still hit a lot of good shots though.'
As for playing 36 on Sunday, Bowden isn't concerned.
'It doesn't bother me any to play 36 holes in one day,' said Bowden. 'We'll get a break for lunch wouldn't we? That is my favorite part of the day. I'm excited about the opportunity and challenge awaiting me on Sunday.'
Matteson was flawless on Saturday with six birdies and no bogeys. He tallied back-to-back birdies twice on the back nine to get in position for a fourth top-10 in his last five starts.
'I think once you start playing well it helps you play well in the future,' said Matteson. 'I'm playing with a lot of confidence right now. I'm making good decisions on the golf course.'
Eric Axley (67) and Ryan Armour (68) share fourth place at 6-under-par 136.
Tjaart van der Walt (70), Scott Petersen (67), Charley Hoffman (69) and Mike Heinen (70) are knotted in sixth place at 4-under-par 138.