Laura Matthews, an assistant coach for the women's team at her alma mater, the University of Georgia, posted a 2-under 70, while her teammate, Lindsay Knowlton, a senior at Ohio State University, chipped in with a 3-over-par 75.
'We're very comfortable with the lead,' said Jackie Little, the captain of the Canadian team. 'All of these players are used to winning.'
Sweden's team of 2004 NCAA runner-up Karin Sjodin (70) and 2004 Ladies British Amateur champion Louise Stahle (71) teamed up for a 3-under 141 and are alone in second place at 3-under-par 285.
The United States squad of reigning U.S. Amateur champion Jane Park (71) and Paula Creamer (76) struggled to a 3-over 147 and dropped to third at minus-2.
The start of the second round was delayed two hours due to lightning in the morning and an overnight storm that left much work to be done to improve the course. The teams were re-paired and play went off both the first and 10th tees of both the River and Ocean courses.
Spain (145) and Colombia (140) are tied for fourth place at 1-over-par 289, while the Korean team, which was in second after the opening round, fell to a 4-over 148 and are alone in sixth at plus-2.
The Japanese team carded their second 3-over 147 in as many days and are in seventh place at 6-over-par 294. Chinese Taipei (152), Belgium (149) and the Russian Federation (150) are knotted in eighth place at 7-over-par 295.