Rod Pampling (65) and Robert Allenby (67) are knotted at minus-9 at Bay Hill Golf Club and Lodge. Dean Wilson, who shared the overnight lead, managed a 2-under 70 on Friday and is alone in fourth place at 8-under-par 136.
Tiger Woods, a four-time winner of this event and the No. 1 player in the world, could not get anything going on Friday. He carded a 1-under 71 and is part of a group tied for 25th place at minus-3.
Woods made the turn at 1 under par thanks to an eagle and a bogey. He ran home a 10-footer for birdie at the 12th, but missed the green with a wedge at the 15th. The reigning Masters and British Open champion chipped 8 feet past, but missed the par-saver.
Woods narrowly missed birdie putts at 16 and 17 and will have his work cut out for him on the weekend if he is to catch the leader.
Glover, who earned his first PGA Tour victory last year in Disney World, started quickly on Friday as he hit a 6-iron to 4 feet to set up birdie at the first. Glover parred his next four holes before he returned to red figures.
He reached the par-5 sixth green in two and two-putted for birdie. At the seventh, Glover holed out from just left of the putting surface. He added a fourth birdie of his round at the ninth from 10 feet. That birdie put Glover at 9 under par for the championship, but trouble loomed for the 26-year- old.
At the par-4 11th, Glover drove well left of the fairway off the tee. He 'decided to be an idiot,' and went for the left side of the putting surface with his second, but splashed into the water. Glover dropped, but failed to get up and down and walked off with a double bogey six.
Glover rebounded at 13 when his wedge approach stopped inside a foot from the hole. He tapped in for birdie, then parred his next two. Glover played strong down the stretch to earn the second-round lead.
At the par-5 16th, Glover hit another poor drive, but got lucky as he had an 8-iron to the green. He two-putted for a birdie from a long distance to join Allenby and Pampling at minus-9.
Glover hit 'probably the best shot all week,' when he laced a 3-iron 8 feet behind the flag. He converted the birdie putt to get to 10 under par, then saved par at 18 after another bad drive to take a piece of the 36-hole lead for the first time in his PGA TOUR career.
'It was a lot like yesterday, a lot of fairways and a lot of greens and I putted well,' said Glover. 'I think 5 under around here two days in a row are great days. The consistency and the fact that I have played solid is definitely a positive.'
Pampling recorded four birdies on the front nine and his 25-footer at the ninth was the only one over 4 feet. The story was the same on the back nine as he posted three birdies with two inside 5 feet and one, at the 17th, from 25 feet.
'It was a good ball-striking day,' acknowledged Pampling. 'The conditions are great out there. I drove it quite nicely and a lot of my iron shots were always around pin-high, which gave me a lot of opportunities to make birdies.'
Allenby, who won three tournaments in Australia at the end of last season, began on the back nine Friday and birdied the 13th and 16th hole. His tee ball plugged in a bunker at 17 and he walked off with a bogey.
That would be the last miscue for Allenby in round two. He kicked in two short birdie putts at one and four, then holed a 15-foot birdie putt at five and a 20-footer at six to get within one of the lead.
'I'm hitting the ball good,' said Allenby, who has not won on the PGA Tour since 2001. 'I'm driving the ball well, hitting a lot of good iron shots and I'm doing everything pretty consistent.'
Corey Pavin (69), Bob Hope Chrysler Classic champion Chad Campbell (71), Harrison Frazar (66) and WGC - Accenture Match Play Championship winner and last week's Honda Classic runner-up Geoff Ogilvy (68) share seventh place at minus-6.
The 36-hole cut fell at 1-over-par 145 and among the notable players who will miss the weekend are: John Daly (146), Colin Montgomerie (148) and reigning U.S. Open champion Michael Campbell (148).