Webb Simpson of Raleigh, North Carolina and Korean Sung Hoon Kang stand just one stroke off the pace following rounds of 69.
Five players -- Juan Candela, Kevin Chappell, Adam Cohan, Dan Doyle and James Sacheck -- are within two strokes of the lead.
After making bogey on the 10th hole to fall back to even par, Kizzire birdied 11 and 12, then parred in on the par-70, 6,575-yard Columbia Country Club layout.
Kizzire, 17, is playing in his first Junior Amateur and had some solid strategy for day one.
'I went out there, trying to make par and just trying to play steady golf,' said Kizzire. 'But then I bogeyed the first hole, and I said this is not my game. I need to shoot as low as I can. I just wanted to play my game and make birdies.'
Simpson, the oldest player in the field who will turn 18 in August, and Kang, 16, both rallied on the back nine. Simpson birdied the 11th, 12th and the par-3, 168-yard 13th hole, while, Kang also birdied the 12th and 13th holes, as well as the par-4, 423-yard 14th hole.
The USGA is making its second visit to Columbia Country Club. In 1921, the course hosted the U.S. Open, won by Jim Barnes. Barnes' nine-stroke victory remains the third-largest in U.S. Open history.
Previous winners of this championship include Tiger Woods, a three-time winner, as well as David Duval, Johnny Miller, Andy North, Scott Simpson and Ken Venturi -- all major championship winners.