Michelle Wie carded a 5-over 76. She finished at 12-over-par 154, in next to last place.
Tjaart van der Walt and Jason Schultz share third place at 7-under-par 135, with Danny Briggs, Fran Quinn and overnight leader Charles Warren one stroke further back at minus-6.
Tambellini started the day three strokes off the pace and did not make much of a move to start his round. He opened with six straight pars at Hillcrest Country Club. He finally got on the board with a birdie at the par-5 16th.
'Making it through the back nine, my front nine, with a decent score was the key,' said Tambellini. 'I made quite a few 10- and 12-footers for par on the front nine and then played really well on the back nine.'
On the front side, the 28-year-old caught fire. He birdied the par-5 third and followed with another at the next to move to 6 under.
Tambellini closed out his round in fine fashion as he birdies each of the final three holes to grab a share of the lead.
'I've felt good all year I just haven't had a good finish,' said Tambellini. 'Now I just hope to play on the weekend like I have the first two days.'
Wilson jumped out of the gate with an eagle on the par-5 second. He climbed to minus-8 with a birdie on the fourth. However, he dropped a shot at the next.
He came back to birdie the eighth, then the 10th to get to 9 under. Wilson stumbled to a bogey at the 13th but recovered to birdie the 15th.
Wilson dropped another shot when he bogeyed No. 16. He was able to climb into a share of the lead with a birdie on the 17th.
'I got off to a great start today,' said Wilson. 'I just kind of relaxed after that. The putt on No. 16 was a big putt for me. It kept me near the top of the leaderboard. Overall, I'm just happy that I'm going to get paid this week.'
Wie opened her round on the back nine and bogeyed two of her first four holes. The 13-year-old then double bogeyed the par-4 15th, but managed to come back with a birdie at the next.
She bogeyed two straight holes from the 18th. She again stopped the skid with birdie, this one coming at the second. However, she dropped one more stroke when she bogeyed the eighth to finish at plus-12.
'I had a lot of fun this week even though I didn't play as well as I wanted to,' said Wie, who hit 10 greens and 10 fairways in the second round. 'I think I learned a lot this week. I think I learned how to play from the thick rough and on fast greens. I improved a lot from yesterday and I'm pretty happy with that.'
Jason Bohn, the runner-up at the Oregon Classic last week, carded a 1-under 70. He stands at 5-under-par 137 where he is joined by Ryan Howison, Daniel Chopra, Johnson Wagner, Brett Wetterich, Bobby Kalinowski and Ryan Palmer.
The cut line fell at even-par 142. Zach Johnson, who won the Utah Classic two weeks ago, was among 15 players to survive on the number.