Jeff Klauk, Dan Olsen, Brian Wilson, Bubba Watson, Jason Dufner, Bill Glasson, Zach Johnson and Brian Kamm share second place at 6-under-par 65.
Glover had a steady opening with four straight pars to begin his day at Glenmaura National Golf Club. He began to make a move on the par-4 fifth, where he rolled in his first birdie of the day. Glover followed that up with an eagle from 10 feet at the next to quickly go from even-par to minus-3.
'The eagle started everything for me,' Glover said. 'It feels good to get off to a good start. I've hit it better, but my wedges were good today and I think I made all six- and eight-footers in the middle of the round.'
The former Clemson Tiger climbed to 4-under with a birdie at the par-4 eighth. He picked up his next birdie at the par-3 11th.
Glover converted back-to-back birdies from the 13th to get to 7-under. However, he stumbled to a bogey on the par-3 15th. Glover closed out his round in style as he birdied the par-4 closing hole. This birdie gave him the lowest round of his 30-start professional career.
Glover's low score was aided by the fact that the opening round was played under lift-clean-and-place rules due to soaking rain the past few weeks.
'When you put your hands on it, you really want it in the fairway,' said Glover, who is searching for his first Nationwide Tour victory. 'When you can lift it, fairways and greens are always the game plan. You want it in the fairway, regardless of whether you hit driver, 3-wood or 2-iron off the tee.'
Klauk started out on fire on the back side of Glenmaura National. He birdied the par-5 10th to begin his day and followed that up with a hole-in-one at the par-3 11th.
'A hole-in-one gets you under-par pretty quick but I had to tell myself I still had 70 holes of golf to play,' said Klauk. 'I thought about making more birdies. The minute you play conservative out here you get passed.'
The 25-year-old followed that up with birdies on Nos. 13 and 15 to get to 5-under through his first six holes. He settled down from there with five straight pars. Klauk birdied the par-4 third before parring his final six holes for an opening-round 65.
Jeff Hart, the 2000 champion here, carded a 5-under-par 66. He shares 10th place with Zoran Zorkic, Jason Bohn, Daniel Chopra, Jay Delsing and Mike Sullivan.
Gary Hallberg, the defending champion, managed just an even-par 71.