Jack and Jack II Nicklaus and Hale and Steve Irwin are tied for second place at 10-under-par 62. Mark and Shaun O'Meara and Larry and Josh Nelson share fourth at minus 9.
This unique format allows fathers and sons to compete together in a scramble. The fathers must have won a major championship and this year there is a grandfather and grandson playing. Arnold Palmer and his grandson Sam Saunders are in the field.
The Langers tallied five birdies in a row to start their round, then closed with four birdies in a row from the 14th. Stefan Langer canned an eight-foot eagle putt at the last to leap ahead of the Nicklaus family and the Irwins.
Now, it's a final-round pairing with the Nicklaus family, something the elder Langer thinks the younger will enjoy.
'We didn't expect to be in the lead, but we got off to a good start,' said Bernhard Langer, who will captain the European side at next year's Ryder Cup. 'It will be an awesome experience for Stefan to play with Jack tomorrow. How many chances will he have to do that?'
The Nicklaus family used a string of seven consecutive birdies to take their share of second place.
'We didn't play great, we played alright,' said Jack Nicklaus, who captained the American side to a tie at last month's Presidents Cup. 'I can't complain with seven birdies at the end.'
The Irwins are hoping to change their history at this event come Sunday. They have finished as runners-up the last two years.
Tom and David Kite are alone in sixth place at 8-under-par 64, followed by Palmer and Saunders and the defending champions, Craig and Kevin Stadler. That pair is tied at minus 7.
Bernhard Langer/Stefan Langer 33-28-61
Jack Nicklaus/Jack Nicklaus II 33-29-62
Hale Irwin/Steve Irwin 32-30-62
Mark O'Meara/Shaun O'Meara 35-28-63
Larry Nelson/Josh Nelson 34-29-63
Tom Kite/David Kite 32-32-64
Craig Stadler/Kevin Stadler 35-30-65
Arnold Palmer/Sam Saunders 36-29-65
Raymond Floyd/Robert Floyd 34-32-66
Dave Stockton/Ron Stockton 35-31-66
Jerry Pate/Wesley Pate 35-31-66
Vijay Singh/Qass Singh 33-33-66
Johnny Miller/Scott Miller 35-31-66
Lee Trevino/Tony Trevino 37-30-67
Lanny Wadkins/Travis Wadkins 37-33-70
Seve Ballesteros/Javier Ballesteros 37-33-70
Full Coverage of the Office Depot Father/Son Challenge