This also ended a streak of consecutive cuts made for Singh, which dated back to last March in The Players Championship.
'I'll probably start a streak next week,' said Singh, in reference to his plans of continuing his relentless schedule next week at the Nissan Open.
Singh's streak of 12 consecutive top-10 finishes carried with it three victories, including last week's title at Pebble Beach.
The Fijian managed a 71 on Thursday on the North Course at Torrey Pines, which was in rotation with the South Course for the first two rounds of the Buick Invitational.
The lengthened South Course, which will host the U.S. Open in 2008, was unforgiving on Friday as Singh, who teed off on No. 10, struggled with two bogeys and a birdie over his first nine holes.
Singh's problems continued on the front side with a pair bogeys from the third.
He found a glimpse of hope with an eagle at the par-5 sixth but was still on the outside looking in coming down the stretch.
Singh had a birdie opportunity at the par-5 ninth, his last, that would have put him on the projected cut line of 1 under par. Singh's putt passed to the right of the hole, however, and just like that the streak was ended with a second-round 73 and an even-par 144 finish.
Singh was two top-10 finishes away from matching Jack Nicklaus' run of 14 consecutive top-10s.