Garcia was eliminated first in the playoff, but used his T-2 finish to jump three spots, from No. 10 to No. 7 in the world.
Fowler played his last 10 holes in 8 under to win his second PGA Tour title and tie his highest previous position in the rankings at No. 9.
But it was Kisner, given his relative lack of experience and success on Tour, who made the biggest leap, vaulting from 123rd to 66th. He will earn an invite to the U.S. Open at Chambers Bay provided he can crack the top 60 on either May 25 or June 15.
Across the world, on a volcanic island nation due East of Madagascar, George Coetzee parlayed his win at the Mauritius Open into a move from 63rd to 52nd.
In less positive news, Adam Scott, who continues to struggle with his putting ahead of next year's anchoring ban, fell out of the top 10 for the first time since July 2012, the week before his British Open collapse at Royal Lytham.
And, finally, in the weekly Tiger Woods watch, the former world No. 1 slipped from 125th to 133rd with a T-69 finish at The Players. He's now sandwiched between K.J. Choi and 24-year-old Englishman Eddie Pepperell.
Here is your OWGR top 10 for the week of May 10: 1. Rory McIlroy, 2. Jordan Spieth, 3. Henrik Stenson, 4. Bubba Watson, 5. Jim Furyk, 6. Justin Rose, 7. Sergio Garcia, 8. Jason Day, 9. Rickie Fowler, 10. Dustin Johnson.